01
Start with the application
What must the antibody recognise? What should it avoid? Which samples, conditions and assay format matter? Those requirements should guide antigen selection and screening criteria from the outset.
Monoclonal antibodies developed with your assay in mind.
We're building Two-To-One Bio to develop and produce monoclonal antibodies for diagnostic assay developers. Our starting point is what your antibody needs to do—in your samples, under your assay conditions.
What we do
Monoclonal antibodies are our focus. Our focus is developing the antibody component for companies building diagnostic assays. We're shaping a hybridoma-based development approach around the intended application—from antigen and immunogen selection to the criteria used to screen and compare candidates.
The problem we solve
A positive signal is only the beginning.
An antibody can recognise the target and still fall short in the intended assay. Cross-reactivity, sample conditions, food processing and compatibility with the assay format all matter. Our approach is to bring those questions into candidate selection, rather than leave them until the end.
02
Keep candidates in contention
An early positive result is a reason to investigate, not the whole basis for selection. Our approach is to retain multiple antibody-producing cell clones for further comparison, so relevant candidates can be evaluated before narrowing the choice.
03
Test what matters to the assay
We aim to assess candidates against relevant cross-reactivity risks, samples and assay conditions. For food allergens, that includes considering how processing may affect recognition. The scope of testing needs to match the intended use.
04
Make the evidence useful
The goal is to document what was tested, under which conditions and where questions remain—giving your team a clearer basis for selecting and evaluating a candidate in its own assay.
Let's talk
What does your next assay need?
We are defining our first antibody targets through conversations with prospective customers. Planning a new assay? Struggling to source a suitable antibody? Working around a limitation your current reagents cannot resolve?
Tell us what you need the antibody to do—and what your current options leave unresolved.
Tell us about the target, the assay format, the samples involved and the performance you need. We're interested in what would make a candidate worth evaluating—and what would rule it out.
No generic sales flow. No automated pitch. Just a direct technical conversation.
Monoclonal antibody focus · Application-led development · Direct technical conversation
Technical notes
Join the Two-To-One Bio Technical Notes
Occasional short notes on monoclonal antibody development and assay performance: antigen selection, screening, cross-reactivity, sample effects, characterisation and updates as Two-To-One Bio takes shape.